A specific carotenoid is required for reconstitution of theRubrivivaxgelatinosusB875 light harvesting complex from its subunit form B820
- 17 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 353 (2) , 151-154
- https://doi.org/10.1016/0014-5793(94)01033-1
Abstract
The core light-harvesting complex B875 from Rubrivivax gelatinosus has been reconstituted from its subunit form B820 with hydroxyspheroidene, the carotenoid which is bound to native B875 antenna. Other carotenoids which are chemically similar to hydroxyspheroidene (spheroidene, spheroidenone, neurosporene and spirilloxanthin) gave only low levels of partial reconstitution. Absorption and circular dichroism spectra of the hydroxyspheroidene-containing, reconstituted B875 were identical with those of original B875 antenna isolated directly from chromatophores, indicating that the two hydroxyspheroidene molecules bind to their native sites within the (αβ)Bchl2, subunit during the reconstitution process. These observations point to a structural role for this carotenoid in determining the architecture of Rv. gelatinosus B875 antenna.Keywords
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