Isolation and renaturation of α2-macroglobulin receptor from diploid human fibroblasts
- 15 August 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 214 (2) , 629-631
- https://doi.org/10.1042/bj2140629
Abstract
Alpha 2-Macroglobulin receptor was extracted from human diploid fibroblasts and purified by affinity chromatography in a single step. The receptor had mol.wt. 125 000 after sodium dodecyl sulphate (SDS)/polyacrylamide-gel electrophoresis. The isolated receptor was separated by SDS/polyacrylamide-gel electrophoresis, transferred on to nitrocellulose sheets and subsequently renatured, as shown by a specific binding test, by incubation with Nonidet P40.This publication has 4 references indexed in Scilit:
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