HPLC of Glycoalkaloids fromSolanum sodomaeum

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Abstract
Glycoalkaloids were extracted from plant material of Solanum sodomaeum L. High-performance liquid chromatographic studies indicated that several glycoalkaloids were present. The sugar moiety of the glycoalkaloids consisted of a mixture of glucose, rhamnose, and galactose. Mass spectral analyses showed that all the glycoalkaloids contained solasodine. Solasonine and solamargine were identified and were present at similar concentrations, representing 67% of the total extracted glycoalkaloids. The extraction yield of the glycoalkaloids from plant material, expressed as g glycoalkaloid/100 g wet weight, was highest for ripe fruit (0.83 ± 0.11), followed by unripe fruit (0.45 ± 0.08), leaves (0.14 ± 0.01), and stems (< 0.04) with progressively decreasing yields. Over a twofold difference in glycoalkaloid yield was observed when ripe fruit of two different districts was extracted.

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