Enhancement of presynaptic calcium current by cysteine string protein
Open Access
- 1 January 2002
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 538 (2) , 383-389
- https://doi.org/10.1113/jphysiol.2001.013397
Abstract
The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic N‐type Ca2+ channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca2+ current amplitude. However, this increase could not be attributed to a change in Ca2+ channel kinetics, voltage dependence, prepulse inactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Secretory vesicle associated endogenous CSP may play an important role in enhancing Ca2+ channel activity at the transmitter release site.Keywords
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