Growth-inhibitory effects of 1,25-dihydroxyvitamin D3 on normal and psoriatic keratinocytes

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Abstract
The effects of 1,25‐dihydroxyvitamin D3 (1,25(OH)2D3) on the growth and DNA synthesis of cultured human keratinocytes obtained from involved and uninvolved psoriatic epidermis and normal epidermis were studied. Treatment with 10‐8m and 10‐7m of 1,25(OH)2D3 inhibited cell growth as follows: 58.5±19.3% and 21.3±13.6% in normal keratinocytes (n=6); 43.8±22.8% and 17.8±12.3% in psoriatic uninvolved keratinocytes (n=4); 51.7±18.2% and 13.2±6.4% in psoriatic involved keratinocytes (n=6). Inhibition was virtually complete at 10‐6m. DNA synthesis was also inhibited by 10‐8m, 10‐7m and 10‐6m of 1,25(OH)2D3 as follows: 70.0±8.3%, 59.0±6.8% and 16.7±4.0%, respectively, in normal keratinocytes (n=3); 78.5±13.5%, 51.5±25.5% and 24.5±21.5%, respectively, in psoriatic uninvolved keratinocytes (n=2); and 69.3±14.5%, 41.3±19.1% and 14.8±11.2%, respectively, in psoriatic involved keratinocytes (n=4). These results indicate that 1,25(OH)2D3 functions as a growth inhibitor for cultured human keratinocytes derived from both normal and psoriatic skin.

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