Ig/EBP (C/EBPγ) is a transdominant negative inhibitor of C/EBP family transcriptional activators
Open Access
- 1 January 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 23 (21) , 4371-4377
- https://doi.org/10.1093/nar/23.21.4371
Abstract
Analysis of cDNA and genomlc clones shows that the murine Ig/EBP (C/EBPγ) gene encodes a small protein with a predicted molecular weight of 16.4 kDa which contains C/EBP family basic and leucine zipper domains but lacks the transcriptional activation domains present in C/EBP (CZEBPa)and NF-IL6 (C/EBPp). In transfectlon assays Ig/EBP is neither an activator nor a repressor of transcription; however, Ig/EBP inhibits the transcriptional ability of NF-IL6 (C/EBPp1) and C/EBP (C/EBPa), acting as a transdominant negative regulator. Thus Ig/EBP resembles LIP, another negative regulator of the C/EBP family, in both structure and transcriptional activity. Of the three known C/EBP family inhibitors, Ig/EBP, LIP and CHOP-10, only g/EBP is ubiquitously expressed. Therefore, Ig/EBP may act as a general buffer for C/EBP activators in many cell types.Keywords
This publication has 44 references indexed in Scilit:
- Targeted disruption of the NF-IL6 gene discloses its essential role in bacteria killing and tumor cytotoxicity by macrophagesCell, 1995
- Reciprocal Regulation of Adipogenesis by Myc and C/EBPαScience, 1992
- More is better: Activators and repressors from the same geneCell, 1992
- A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the sam mRNACell, 1991
- Molecular cloning of cDNA and a chromosomal gene encoding GPE1‐BP, a nuclear protein which binds to granulocyte colony‐stimulating factor promoter element 1FEBS Letters, 1991
- A naturally occurring truncated form of FosB that inhibits Fos/Jun transcriptional activityCell, 1991
- IL-6DBP, a nuclear protein involved in interleukin-6 signal transduction, defines a new family of leucine zipper proteins related to CEBPCell, 1990
- Jun-B differs in its biological properties from, and is a negative regulator of, c-JunCell, 1989
- Scissors-Grip Model for DNA Recognition by a Family of Leucine Zipper ProteinsScience, 1989
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979