Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 47 (2) , 199-202
- https://doi.org/10.1007/bf00273202
Abstract
The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell.Keywords
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