EVIDENCE THAT THE REPELLENT RECEPTOR FORM OF SENSORY RHODOPSIN I IS AN ATTRACTANT SIGNALING STATE*
- 1 December 1991
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 54 (6) , 1023-1026
- https://doi.org/10.1111/j.1751-1097.1991.tb02125.x
Abstract
The lifetime of the Halobacterium halobium sensory rhodopsin I (SR-I) photocycle intermediate S373 was modulated by incorporating retinal analogs into SR-I apoprotein in vitro and in vivo. Photocycles by SR-I analog pigments exhibit the same reaction scheme and similar formation rates, but different decay rates, of their S373-like species as monitored by flash spectroscopy in membrane vesicle suspensions. The attractant receptor signaling efficiencies determined by physiological measurements are proportional to the lifetimes of the S373-like intermediates, indicating that S373 is a physiological active conformation (signaling state) of the receptor. A model incorporating this finding into the SR-I photocycle is presented.Keywords
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