Total Internal Reflection Fluorescence Microscopy in Cell Biology
Top Cited Papers
Open Access
- 1 November 2001
- Vol. 2 (11) , 764-774
- https://doi.org/10.1034/j.1600-0854.2001.21104.x
Abstract
Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell‐substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope.Keywords
This publication has 52 references indexed in Scilit:
- Protein Kinase C-Dependent Supply of Secretory Granules to the Plasma MembraneBiochemical and Biophysical Research Communications, 2001
- Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence MicroscopyThe Journal of cell biology, 2000
- Super-resolution measurements with evanescent-wave fluorescence excitation using variable beam incidenceJournal of Biomedical Optics, 2000
- Simultaneous Imaging of Individual Molecules Aligned Both Parallel and Perpendicular to the Optic AxisPhysical Review Letters, 1998
- Time‐lapse total internal reflection fluorescence video of acetylcholine receptor cluster formation on myotubesDevelopmental Dynamics, 1994
- Annexin IV Reduces the Rate of Lateral Lipid Diffusion and Changes the Fluid Phase Structure of the Lipid Bilayer When It Binds to Negatively Charged Membranes in the Presence of CalciumBiochemistry, 1994
- Binding of proteins to specific target sites in membranes measured by total internal reflection fluorescence microscopyBiochemistry, 1990
- Evanescent field excitation of fluorescence by epi-illumination microscopyApplied Optics, 1989
- Fluorescence emission at dielectric and metal-film interfacesJournal of the Optical Society of America B, 1987
- Cell-substrate contacts illuminated by total internal reflection fluorescence.The Journal of cell biology, 1981