Regulation of polyomavirus transcription by large tumor antigen.

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Abstract
The regulation of viral transcription by the large number antigen was analyzed in cells infected by several viable deletion and insertion mutants of polyomavirus. Deletion of the early promoter "TATA box" and associated large tumor antigen binding site has only a small effect on the balance of early and late mRNA. Transcription of a polyomavirus containing a heterologous adenovirus promoter in place of the normal TATA box and cap sites is regulated by the large tumor antigen. Repression of polyomavirus early transcription cannot occur simply by binding of the large tumor antigen to DNA sequences at the site of transcription initiation and must involve the interaction of the large tumor antigen binding at other sites.