Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae.
- 1 March 1996
- journal article
- Vol. 142 (3) , 749-59
Abstract
The soh1, soh2 and soh4 mutants were isolated as suppressors of the temperature-dependent growth of the hyperrecombination mutant hpr1 of Saccharomyces cerevisiae. Cloning and sequence analysis of these suppressor genes has unexpectedly shown them to code for components of the RNA polymerase II transcription complex. SOH2 is identical to RPB2, which encodes the second largest subunit of RNA polymerase II, and SOH4 is the same as SUA7, encoding the yeast transcription initiation factor TFIIB. SOH1 encodes a novel 14-kD protein with limited sequence similarity to RNA polymerases. Interestingly, SOH1 not only interacts with factors involved in DNA repair, but transcription as well. Thus, the Soh1 protein may serve to couple these two processes. The Soh1 protein interacts with a DNA repair protein, Rad5p, in a two-hybrid system assay. Soh1p may functionally interact with components of the RNA polymerase II complex as suggested from the synthetic lethality observed in soh1 rpb delta 104, soh1 soh2-1 (rpb2), and soh1 soh4 (sua7) double mutants. Because mutations in SOH1, RPB2 and SUA7 suppress the hyperrecombination phenotype of hpr1 mutants, this suggests a link between recombination in direct repeats and transcription.This publication has 31 references indexed in Scilit:
- A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coliPublished by Elsevier ,2003
- Genetic control of intrachromosomal recombinationBioEssays, 1995
- RAD25 is a DMA helicase required for DNA repair and RNA polymerase II transcriptionNature, 1994
- DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase IINature, 1994
- Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repairCell, 1993
- A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeastCell, 1993
- The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivoCell, 1992
- RNA polymerase II C-terminal repeat influences response to transcriptional enhancer signalsNature, 1990
- A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatasesCell, 1989
- Functional redundancy and structural polymorphism in the large subunit of RNA polymerase IICell, 1987