Adenosine 3′,5′-Monophosphate (cAMP)-Binding Proteins and cAMP-Dependent Protein Kinases in Bovine Adrenal Cortical Cell Plasma Membrane*
- 1 July 1981
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 109 (1) , 301-306
- https://doi.org/10.1210/endo-109-1-301
Abstract
A cAMP-binding protein and cAMP-dependent protein kinase have been identified and partially characterized in bovine adrenocortical plasma membranes. [3H]cAMP binding to the plasma membrane preparation demonstrates both high (Kª = 4.2 × 109 M-1) and low (Kª = 1.3 × 108 M-1) affinity binding sites. cAMP-dependent protein kinase was demonstrated using both bovine histone and endogenous substrates. The detergentdependent solubilization characteristics of this cAMP-dependent protein kinase indicate that it is an integral membrane protein.Keywords
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