Changes in Calcium Levels in Cell Walls during Elongation of Oat Coleoptile Sections

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Abstract
Calevelsinoatcoleoptile sections were determined on intact sections and cell wall fractions by atomic absorption spectrophotometry. The measurements were made over an 18-hour period in both the presence and absence of 3.5 mg/liter of indoleacetic acid and in both the presence and absence of 200 mg/liter Ca. Ca actively accumulated in the intact sections and showed a 2-fold increase in the walls over the 18-hour period if Ca was present in the medium. Indoleacetic acid had no effect on this accumulation. These results, based on determinations of Ca rather than Ca45, provide additional evidence that the mode of action of auxin is not through effects upon Ca levels in cell walls.