Comparison of Various Staining Procedures in the Identification of Hepatozoon canis Gamonts
- 1 September 1988
- journal article
- Published by Wiley in Veterinary Clinical Pathology
- Vol. 17 (3) , 63-65
- https://doi.org/10.1111/j.1939-165x.1988.tb00492.x
Abstract
Summary: The staining characteristics of gamonts of Hepatozoon canis in peripheral blood smears were evaluated. Three stains, Diff‐Quik stain, Giemsa stain and a modification of the naphthol‐ASD‐chloroacetate esterase stain, were compared. The staining characteristics of the infected cell and the parasite were different in each stain used. The modified naphthol‐ASD‐chloroacetate esterase stain showed the most difference between the infected and noninfected cells, as well as a difference in staining characteristics of the parasite nucleus from those of the host nucleus. Although the parasite was identified with all stains, a definitive diagnosis was more easily obtained with the naphthol‐ASD‐chloroacetate esterase stain.Keywords
This publication has 1 reference indexed in Scilit:
- Alterations in the Plasma Membranes of Hepatozoon-Infected Snake Erythrocytes as Evidenced by Differential StainingJournal of Parasitology, 1984