The uptake of 35S into rat tissues after injection of [35S]methionine

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Abstract
The specific radioactivity of rat tissues was measured at different times after the intraperitoneal injection of L-[S35] methionine into rats. The pattern of uptake of S35 in order of decreasing radioactivity was: liver, kidney, spleen, lung, heart, brain and muscle. The pattern of uptake of S35 from DL-[S35]-methionine differed from that of the L isomer for liver and kidney, but was similar for other tissues. The specific activities of lipid, protein and acid-soluble fractions of brain and liver tissue were measured. There was a relatively rapid uptake of S35 from the acid-soluble into the protein fraction of the brain. The rate of uptake of S35 into the tissues depends on constitutional factors that were found to vary in rats of 2 different genetic strains, but with animals of uniform strain consistent results were obtained. Values are given for the total sulfur content of rat tissues, and for the sulfur content of the protein, lipid and acid-soluble fractions of liver and brain.