Studies on the Removal of Staphylococcus Aureus from Milk-Contact Surfaces by Ultrasonic Cleaning Methods

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Abstract
The test organism was incorporated into soil menstrua (modified Ringer Solution and homogenized milk), dried down upon various milk-contact surfaces, removed by ultrasonic cleaning treatments, and assayed by submersion in Staphylococcus Medium No. 110, Bacto-Strip contact, plastic replica-embedding and staining, Geiger counting, and autoradiographic techniques. Soil buildup can be a problem in ultrasonic cleaning if the initial deposits are not thoroughly removed. The rough surfaces show greater capacity for soil build-up. The type of surface roughness also influences the pattern of subsequent growth of the residual organisms. The type of cleaning solution and time of ultrasonic irradiation exerted a definite influence on cleaning efficiency; however, specimen configuration and type of bacterial soil menstrum were of greater importance.