Multiplex analysis of expression of three IFNβ-induced genes in antibody-positive MS patients
- 14 February 2006
- journal article
- research article
- Published by Wolters Kluwer Health in Neurology
- Vol. 66 (3) , 444-446
- https://doi.org/10.1212/01.wnl.0000196467.71646.72
Abstract
Some interferon beta (IFNβ)–treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed real-time multiplex reverse transcriptase PCR to measure expression of three IFNβ-inducible genes to directly assess IFNβ bioactivity in patients with neutralizing antibodies (NAbs). The three genes responded in tandem. Correlation of NAb level with bioactivity at low/moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNβ bioactivity is most reliable.Keywords
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