Centrifugation-Shell Vial Technique for Rapid Detection of Mediterranean Spotted Fever Rickettsia in Blood Culture
- 1 February 1989
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 40 (2) , 197-199
- https://doi.org/10.4269/ajtmh.1989.40.197
Abstract
The shell vial technique for isolation of cytomegalovirus was adapted to detect Rickettsia conorii in blood culture using human fibroblast monolayers. The inoculation was performed with low speed centrifugation and the rickettsiae demonstrated by immunofluorescence 24–120 hr after inoculation. R. conorii was identified in 11 of 13 patients with Mediterranean spotted fever in 48–72 hr.This publication has 3 references indexed in Scilit:
- Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell culturesJournal of Clinical Microbiology, 1987
- Correlation of rickettsial titers, circulating endotoxin, and clinical features in Rocky Mountain spotted feverArchives of internal medicine (1960), 1983
- Centrifugation of Rickettsiae and Viruses Onto Cells and Its Effect on InfectionExperimental Biology and Medicine, 1960