Cytobiochemical characterization ofAspergillus terreus17p utilizing various carbon substrates

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Abstract
The thermotolerant mycelial fungus,Aspergillus terreus17p, was shown to be capable of utilizing various carbon substrates, e.g., cellulose, glucose, hexadecane, methanol. In all cases the fungal hyphae cytoplasm contained microbodies differing in morphology with a carbon source. A cytochemical reaction with 3,3‐diaminobenzidine revealed catalase within microbodies. A comparative enzymatic analysis was carried out for cell‐free extract from fungi grown on various carbon substrates. The activities of the enzymes (alcohol oxidase, formaldehyde‐ and formate dehydrogenases, dihydroxyacetone synthase and dihydroxyacetone kinase) catalyzing methanol dissimilation and assimilation were estimated in the methanol‐grown mycelium. Maximum values of hexokinase and glucose‐6‐phosphate dehydrogenase activities were observed in hyphae cultivated with glucose and cellulose. Synthesis of cytochrome P‐450 and oxidase of higher fatty alcohols involved inn‐alkane dissimilation is induced by hexadecane. Activities of the citric acid cycle enzymes, fumarase and isocitrate dehydrogenase, were not essentially influenced by the nature of carbon substrates.