Uranium Reduction by Desulfovibrio desulfuricans Strain G20 and a Cytochrome c 3 Mutant
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (6) , 3129-32
- https://doi.org/10.1128/aem.68.6.3129-3132.2002
Abstract
Previous in vitro experiments with Desulfovibrio vulgaris strain Hildenborough demonstrated that extracts containing hydrogenase and cytochrome c 3 could reduce uranium(VI) to uranium(IV) with hydrogen as the electron donor. To test the involvement of these proteins in vivo, a cytochrome c 3 mutant of D. desulfuricans strain G20 was assayed and found to be able to reduce U(VI) with lactate or pyruvate as the electron donor at rates about one-half of those of the wild type. With electrons from hydrogen, the rate was more severely impaired. Cytochrome c 3 appears to be a part of the in vivo electron pathway to U(VI), but additional pathways from organic donors can apparently bypass this protein.Keywords
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