Purification and characterization of the N‐terminal domain of galectin‐4 from rat small intestine
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3) , 169-172
- https://doi.org/10.1016/0014-5793(95)00025-5
Abstract
Using affinity chromatography on lactose-agarose, five beta-galactoside binding lectins of 14 to 20 kDa were detected in the rat small intestinal mucosa. The prominant proteins of 17 and 19 kDa were purified to homogeneity by 2D-electrophoresis. Direct N-terminal sequencing of the 17 kDa protein and intrachain sequencing of the 19 kDa protein produced sequences which are part of the N-terminal domain of the L-36/galectin-4. A rabbit polyclonal antibody was raised against the 19 kDa lectin, which specifically recognized the 17 and 19 kDa lectins and detected a related 36 kDa protein in human undifferentiated HT29 cells.Keywords
This publication has 17 references indexed in Scilit:
- Purification and Partial Amino Acid Sequence of Fuctinin, an Endogenous Inhibitor of Fucosyltransferase ActivitiesEuropean Journal of Biochemistry, 1994
- Effects of endogenous soluble β-galactoside binding lectins and protein inhibitor of fucosyltransferase on the enzymes involved in the intestinal fucosylation processBiochemical and Biophysical Research Communications, 1992
- Nutritional and developmental regulation of glycosylation processes indigestive organsBiochimie, 1992
- Soluble lactose-binding vertebrate lectins: a growing familyBiochemistry, 1989
- Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaAnalytical Biochemistry, 1987
- The human colon carcinoma cell lines HT-29 and Caco-2: Two in vitro models for the study of intestinal differentiationBiochimie, 1986
- Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocelluloseJournal of Biochemical and Biophysical Methods, 1984
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- A rapid, sensitive, and specific method for the determination of protein in dilute solutionAnalytical Biochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970