Histochemically Demonstrable Changes in Lactate and Succinate Dehydrogenases in Normal Rabbit Synovial Membrane after Injection of Osmium

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Abstract
Summary The changes in lactate dehydrogenase and succinate dehydrogenase activities caused by osmium tetroxide were examined histochemically in normal synovial membrane of the rabbit. The animals were albino rabbits, and they were 18 altogether. 0.3 ml. of 1 % OsO4 solution was injected, i. a., in the right knee. The left knee joint served as a control. One day after the injection, decrease in the enzyme activities was observed around the osmium particles deep in the synovial tissue as a sign of necrosis. Enzyme activity, stronger than normally, was seen after one week in the newly formed granulous tissue. It appeared both in fibroblasts and foreign body giant cells. Because lactate and succinate dehydrogenases indicate the changing energy production in traumatized tissue, these enzymes thus demonstrate the onset of the regeneration process one week after the injection of OsO4.

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