The Interrelation of the Amount of V-factor and the Amount of Air Necessary for Growth of Hemophilus influenzae Type b in Certain Media

Publisher Website
Google Scholar
Abstract
H. influenzae was cultivated under conditions in which the supply of air and the amt. of the accessory growth factor V were varied. The supply of air was increased by rotating test-tube cultures during incubation or by exposing a relatively large surface of broth to the air. The increase in the V-substance was brought about by the addition of yeast extract. The bacteria were also grown in the presence of fresh rabbit serum which inhibits or destroys the action of the V-substance. When certain media are kept well aerated the bacilli are able to grow in the presence of less V than would otherwise be required and vice versa, if the V-factor is increased, growth will occur with less aeration.

This publication has 1 reference indexed in Scilit: