Screening of Microorganisms ProducingD-β-Acetylthioisobutyric Acid from MethylDL-β-Acetylthioisobutyrate

1 January 1992

journal article
Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry

Vol. 56 (8) , 1252-1256
https://doi.org/10.1271/bbb.56.1252

Abstract

Microorganisms producing D-β-acetylthioisobutyric acid from methyl D-β-acetylthioisobutyrate were screened from stock cultures. The D-β-acetylthioisobutyric acid-producing ability was found in 15 strains belonging to the genera Pseudomonas, Agrobacterium, Enterobacter, Cellulomonas, Rhodococcus, Brevibacterium, and Torulopsis. A strain of Pseudomonas fluorescens, IFO 3081, was selected as the best microorganism. The cells having activity (558 units/g of dry cells) could be easily prepared by cultivation at 25°C at pH 6.6 for 24 hr in a glucose-containing medium. The D-form of methyl DL-β-acetylthioisobutyrate was selectively hydrolyzed with the cells so that D-β-acetylthioisobutyric acid (97.2% enantiomeric excess) was produced in a high yield.

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