The metalloclusters of carbon monoxide dehydrogenase/acetyl-CoA synthase: a story in pictures
- 18 June 2004
- journal article
- review article
- Published by Springer Nature in JBIC Journal of Biological Inorganic Chemistry
- Vol. 9 (5) , 511-515
- https://doi.org/10.1007/s00775-004-0563-y
Abstract
Eight Ni proteins are known and three of these, CO dehydrogenase (CODH), acetyl-CoA synthase (ACS), and hydrogenase, are Ni-Fe-S proteins. In the last three years, the long-awaited structures of CODH and ACS have been solved. The bioinorganic community was shocked, as the structures of the active sites of CODH and ACS, the C- and A-cluster, respectively, which each had been predicted to consist of a [Fe4S4] cluster bridged to a single Ni, revealed unexpected compositions and arrangements. Crystal structures of ACS revealed major differences in protein conformation and in A-cluster composition; for example, a [Fe4S4] cluster bridged to a binuclear center in which one of the metal binding sites was occupied by Ni, Cu, or Zn. Recent studies have revealed Ni-Ni to be the active state, unveiled the source of the heterogeneity that had plagued studies of CODH/ACS for decades, and produced a metal-replacement strategy to generate highly active and nearly homogeneous enzyme.Keywords
This publication has 22 references indexed in Scilit:
- Computational Studies on the A Cluster of Acetyl-Coenzyme A Synthase: Geometric and Electronic Properties of the NiFeC Species and Mechanistic ImplicationsJournal of the American Chemical Society, 2003
- Inactivation of Acetyl-CoA Synthase/Carbon Monoxide Dehydrogenase by CopperJournal of the American Chemical Society, 2003
- Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open α subunits of acetyl-CoA synthase/carbon monoxide dehydrogenaseNature Structural & Molecular Biology, 2003
- Nickel in Subunit β of the Acetyl-CoA Decarbonylase/Synthase Multienzyme Complex in MethanogensJournal of Biological Chemistry, 2003
- Rapid Kinetic Studies of Acetyl-CoA Synthesis: Evidence Supporting the Catalytic Intermediacy of a Paramagnetic NiFeC Species in the Autotrophic Wood−Ljungdahl PathwayBiochemistry, 2002
- Crystal Structure of a Carbon Monoxide Dehydrogenase Reveals a [Ni-4Fe-5S] ClusterScience, 2001
- Channeling of Carbon Monoxide during Anaerobic Carbon Dioxide FixationBiochemistry, 2000
- Evidence of a Molecular Tunnel Connecting the Active Sites for CO2Reduction and Acetyl-CoA Synthesis in Acetyl-CoA Synthase fromClostridiumthermoaceticumJournal of the American Chemical Society, 1999
- Undetectable Intracellular Free Copper: The Requirement of a Copper Chaperone for Superoxide DismutaseScience, 1999
- Heterogeneous nickel-iron environments in carbon monoxide dehydrogenase from Clostridium thermoaceticumJournal of the American Chemical Society, 1993