Promoter Sequences Involved in Transforming Growth Factor β1 Gene Induction in HaCaT Keratinocytes after Gamma Irradiation
- 1 March 2002
- journal article
- Published by Radiation Research Society in Radiation Research
- Vol. 157 (3) , 249-255
- https://doi.org/10.1667/0033-7587(2002)157[0249:psiitg]2.0.co;2
Abstract
Gault, N., Vozenin-Brotons, M. C., Calenda, A., Lefaix, J. L. and Martin, M. T. Promoter Sequences Involved in Transforming Growth Factor β1 Gene Induction in HaCaT Keratinocytes after Gamma Irradiation. Radiat. Res. 157, 249 – 255 (2002). Transforming growth factor beta 1 (TGFB1) is a cytokine involved in the development of both acute and late cutaneous radiation syndromes. We previously demonstrated that ionizing radiation induces TGFB1 expression in vivo in pig skin within a few hours. The purpose of the present study was to develop an in vitro human model to identify the mechanisms of this early activation. Accordingly, human HaCaT keratinocytes were irradiated with a single dose of 20 Gy. First, radiation-induced TGFB1 overexpression was checked at both the transcriptional and transductional levels in HaCaT cells. Then electrophoretic mobility shift assays (EMSA) and transient transfection with various TGFB1 promoter constructs were used to identify the sequences involved in regulating this ...Keywords
This publication has 30 references indexed in Scilit:
- TGF-β1 and radiation fibrosis: a master switch and a specific therapeutic target?International Journal of Radiation Oncology*Biology*Physics, 2000
- NF-κB and AP-1 are responsible for inducibility of the IL-6 promoter by ionizing radiation in HeLa cellsInternational Journal of Radiation Biology, 2000
- Transcriptional Activation of Transforming Growth Factor β1 and Its Receptors by the Kruppel-like Factor Zf9/Core Promoter-binding Protein and Sp1Journal of Biological Chemistry, 1998
- Coactivation of AP-1 activity and TGF-β1 gene expression in the stress response of normal skin cells to ionizing radiationOncogene, 1997
- Expression of Transforming Growth Factor-β1 in Mouse Skin During the Acute Phase of Radiation DamageInternational Journal of Radiation Biology, 1995
- Repression of the transforming growth factor-beta 1 gene by the Wilms' tumor suppressor WT1 gene productMolecular Endocrinology, 1994
- Early alterations in extracellular matrix and transforming growth factor β gene expression in mouse lung indicative of late radiation fibrosisInternational Journal of Radiation Oncology*Biology*Physics, 1994
- Temporal Modulation of TGF-β1 and β-Actin Gene Expression in Pig Skin and Muscular Fibrosis after Ionizing RadiationRadiation Research, 1993
- The AP-1 sequence is necessary but not sufficient for phorbol induction of collagenase in fibroblastsBiochemistry, 1991
- Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.The Journal of cell biology, 1988