Evidence for a membrane skeleton in higher plants
- 8 March 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (3) , 313-316
- https://doi.org/10.1016/0014-5793(93)80536-4
Abstract
A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (Mr> 100 kDa). One of these polypeptides (Mr ~ 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioningKeywords
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