Inhibition of HIV-1 Replication in Lymphocytes by Mutants of the Rev Cofactor eIF-5A
- 29 March 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5257) , 1858-1860
- https://doi.org/10.1126/science.271.5257.1858
Abstract
Eukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor required for the function of the human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the HIV-1 Rev response element: Rev complex. Both mutants were constitutively expressed in human T cells. When these T cells were infected with replication-competent HIV-1, virus replication was inhibited. The eIF-5AM13 and eIF-5AM14 proteins blocked Rev trans-activation and Rev-mediated nuclear export.Keywords
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