Multiplicity of the Components of Rat Kidney Antigen Responsible for the Localization of Antirat Kidney Antibodies

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Abstract
Summary: Heating and treatment with 60% trichloroacetic acid were shown to affect various rat kidney antigens differently. Heat treatment of the saline insoluble sediment of rat kidney at 100°C for 30 min at pH 6 caused an important but not total loss in its ability to absorb localizing iodinated antirat kidney antibody. Antibody so absorbed and subsequently eluted still localized in kidney. The comparison of this eluate with the eluate obtained from unheated sediment revealed that some of the antigens are more heat stable than others. Further strong evidence for the multiple nature of kidney antigens responsible for fixing antikidney antibodies in vivo was the fact that heated or unheated trypsin digest of kidney sediment neutralized localizing antibodies differently in a qualitative manner. Trichloroacetic acid (60% TCA) dissolved a large part of kidney sediment and the dissolved material precipitated on dilution with water. The TCA soluble and insoluble materials were able to absorb part of the in vivo localizing antikidney antibodies from I131 G-anti-RK. Assay of the antibody eluted subsequently indicated the existence of antigenic components with different degrees of stability against TCA. Neutralizing properties of trypsin digests of TCA-treated materials were compared with those of digests of the original kidney sediment. The neutralization curves were different in shape in each case indicating that only some components were TCA stable.