Distribution of butylated hydroxyanisole and its conjugates in the tissues of rats.

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Abstract
The distribution of butylated hydroxyanisole (BHA) and its conjugates was investigated in the liver, kidney, pancreas, plasma and urine 1 and 3 h after oral administration of 3H-labeled BHA to rats. The total radioactivities in the tissues were in the order kidney > liver > plasma > pancreas. BHA-S (sulfate-conjugate) and BHA-G (glucuronide-conjugate) could be separated clearly by paper electrophoresis. The radioactivities of BHA and its conjugates separated by electrophoresis were determined, and the proportions of BHA, BHA-G and BHA-S were calculated. Unchanged BHA was mainly found in the liver and pancreas. The proportion of BHA-G increased in the order urine > kidney > plasma > pancreas > liver; that of BHA-S increased in the order plasma > kidney > pancreas > liver > urine. BHA-G was the major metabolite in urine, and BHA-S was only a minor metabolite, but a considerable amount of BHA-S was found in plasma. Whether BHA-S administered i.p. or i.v. is converted into the corresponding glucuronide to be excreted in urine was studied. No significant amount of BHA-S was converted into BHA-G under the conditions used.