Photosynthetic Hydrogen Evolution by Spinach Chloroplasts Coupled to a Clostridium Hydrogenase

Open Access
Publisher Website
Google Scholar
Abstract
Photosystem I activity of isolated chloroplasts has been coupled to photosynthetic hydrogen evolution via methylviologen and a Clostridium hydrogenase. Rates of 125 μmol hydrogen evolved per mg chlorophyll and hour have been obtained. The conditions for such optimal activity suggest that a closed thylakoid vesicle is required in order to prevent side-and backreactions of reduced methylviologen, which compete with and reduce hydrogen evolution.