3D structure of the influenza virus polymerase complex: Localization of subunit domains
- 22 December 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (1) , 308-313
- https://doi.org/10.1073/pnas.0307127101
Abstract
The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP-mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.Keywords
This publication has 36 references indexed in Scilit:
- Mutations in the N-Terminal Region of Influenza Virus PB2 Protein Affect Virus RNA Replication but Not TranscriptionJournal of Virology, 2003
- Identification of Hsp90 as a Stimulatory Host Factor Involved in Influenza Virus RNA SynthesisJournal of Biological Chemistry, 2002
- A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Inhibits Endonucleolytic Cleavage of Capped RNAsJournal of Virology, 2002
- Crystal Structures of Active and Inactive Conformations of a Caliciviral RNA-dependent RNA PolymeraseJournal of Biological Chemistry, 2002
- PA Subunit from Influenza Virus Polymerase Complex Interacts with a Cellular Protein with Homology to a Family of Transcriptional ActivatorsJournal of Virology, 2001
- Mutational analysis of the influenza virus A/Victoria/3/75 PA protein: studies of interaction with PB1 protein and identification of a dominant negative mutantJournal of General Virology, 1996
- Xmipp: An Image Processing Package for Electron MicroscopyJournal of Structural Biology, 1996
- SPIDER and WEB: Processing and Visualization of Images in 3D Electron Microscopy and Related FieldsJournal of Structural Biology, 1996
- Structure of influenza virus ribonucleoprotein particles. II. Purified RNA-free influenza virus ribonucleoprotein forms structures that are indistinguishable from the intact influenza virus ribonucleoprotein particlesJournal of General Virology, 1995
- Are the 5′ ends of influenza viral mrnas synthesized in vivo donated by host mRNAs?Cell, 1979