Redox ratio of mitochondria as an indicator for the response of photodynamic therapy
- 1 January 2004
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 9 (4) , 772-778
- https://doi.org/10.1117/1.1760759
Abstract
The effect of photodynamic therapy (PDT) treatment on the metabolic state of tumor mitochondria is investigated by imaging of tumor redox status. PDT is performed using the photosensitizer pyropheophorbide-2-deoxyglucosamide (Pyro-2DG), which utilizes the glucose import pathway. It is found that Pyro-2DG-induced PDT resulting in a highly oxidized state of tumor mitochondria. This is determined from the redox ratio changes derived from the intrinsic oxidized flavoprotein (Fp) and reduced pyridine nucleotide (PN) [i.e., reduced nicotinamide adenine dinucleotide (NADH)] fluorescence signals observed using a cryoimager. Thus, the redox ratio is a sensitive indicator for providing reliable and informative measurements of PDT-induced tissue damage. In the PDT treated region of the tumor, highly oxidized flavoprotein and diminishing NADH fluorescence is detected, suggesting that flavoprotein and NADH are oxidized by singlet oxygen produced in the photosensitization process. © 2004 Society of Photo-Optical Instrumentation Engineers.Keywords
This publication has 24 references indexed in Scilit:
- Direct Near-infrared Luminescence Detection of Singlet Oxygen Generated by Photodynamic Therapy in Cells In Vitro and Tissues In Vivo¶Photochemistry and Photobiology, 2002
- The role of apoptosis in response to photodynamic therapy: what, where, why, and howPhotochemical & Photobiological Sciences, 2002
- Basic principles of photodynamic therapyJournal of Porphyrins and Phthalocyanines, 2001
- The effect of hypericin and hypocrellin-A on lipid membranes and membrane potential of 3T3 fibroblastsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1999
- Photodynamic therapy: A mitochondrial inducer of apoptosisCell Death & Differentiation, 1999
- Rapid cytochrome c release, activation of caspases 3, 6, 7 and 8 followed by Bap31 cleavage in HeLa cells treated with photodynamic therapyFEBS Letters, 1998
- Photodynamic TherapyJNCI Journal of the National Cancer Institute, 1998
- Mitochondrial photodamage and PDT-induced apoptosisJournal of Photochemistry and Photobiology B: Biology, 1998
- The Role of Subcellular Localization in Initiation of Apoptosis by Photodynamic TherapyPhotochemistry and Photobiology, 1997
- HOW DOES PHOTODYNAMIC THERAPY WORK?Photochemistry and Photobiology, 1992