Characterization of initiation factor 3 from wheat germ. 2. Effect of polyclonal and monoclonal antibodies on activity
- 4 June 1985
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 24 (12) , 2928-2931
- https://doi.org/10.1021/bi00333a017
Abstract
Rabbit polyclonal antibodies to wheat germ initiation factor 3 (eIF-3) were obtained and were shown to react strongly with 4 of the 10 subunits of eIF-3 (pp116, pp87, pp56, and pp36). Two mouse monoclonal antibodies were obtained, one of which reacts specifically with pp87 and one of which reacts specifically with pp36. Highly purified anti-pp87 has no effect on the activity of eIF-3. Highly purified polyclonal antibodies and anti-pp36 inhibit the ability of eIF-3 to support polypeptide synthesis in vitro and the ability of eIF-3 to support mRNA binding to 40S ribosomal subunits. These results provide additional evidence that pp116, pp87, and pp36 are in exposed positions in the eIF-3 particle and that pp36 is essential for activity.This publication has 1 reference indexed in Scilit:
- One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatographyJournal of Immunological Methods, 1982