Characterization of IS Xax1 , a Novel Insertion Sequence Restricted to Xanthomonas axonopodis pv. phaseoli (Variants fuscans and non-fuscans) and Xanthomonas axonopodis pv. vesicatoria

Abstract
IS Xax1 is a novel insertion sequence belonging to the IS 256 and Mutator families. Dot blot, Southern blot, and PCR analyses revealed that IS Xax1 is restricted to Xanthomonas axonopodis pv. phaseoli (variants fuscans and non-fuscans) and X. axonopodis pv. vesicatoria strains. Directed AFLP also showed that a high degree of polymorphism is associated with IS Xax1 insertion in these strains.

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