Antigen shedding by human breast-cancer cells in vitro and in vivo
Open Access
- 30 April 1978
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 37 (5) , 776-779
- https://doi.org/10.1038/bjc.1978.115
Abstract
Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls.Keywords
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