Interphase Nuclei from Lemon-Fruit Tissue

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Abstract
Interphase nuclei were examined in living epidermal cells of intact juice sacs from mature lemon fruits. A definitive halo enveloping each nucleolus was observed. Evidence is presented which substantiates this perinucleolar halo as a definitive nuclear region and not as an optical ring resulting from diffraction about the perimeter of a disk. Interphase nuclei were also examined in mature non-growing and excised lemon fruit tissue growing in vitro. Definitive refractile bodies become evident in the nucleoli of the growing tissue; these manifest distinct birefringence under polarizing microscopy. The presence of anisotropic material in nucleoli of interphase nuclei of apical buds from lemon trees, in stem and root tissues from germinating lemon seedlings, and in vesicle stalks from freshly picked mature lemon fruits shows that the birefringent bodies in the nucleoli of the excised fruit tissue are not abnormal characteristics of cells grown in vitro or of aging processes. Anisotropic nucleolar material was also observed in germinating seedlings of plant genera other than Citrus, thus showing that nucleolar birefringence is not peculiar to Citrus.

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