The complete nucleotide sequence of the glutamine synthetase gene (glnA) of Bacillus subtilis
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 57 (1) , 81-86
- https://doi.org/10.1016/0378-1097(89)90151-1
Abstract
The glutamine synthetase (GS) gene from Bacillus subtilis PCI 219 was cloned in Escherichia coli using the vector pBR329. A plasmid, pSGS2, was isolated from a glnA+ transformant and the cloned GS gene was found to be located in a 3.6 kb DNA fragment. The nucleotide sequence of a 1.8 kb segment encoding the GS was determined. This segment showed an open reading frame which would encode a polypeptide of 444 amino acids. The amino acid sequence of this GS gene product has higher homology with that of the Clostridium acetobutylicum GS than that of the E. coli GS.Keywords
This publication has 0 references indexed in Scilit: