Acaricide Susceptibility in the Lone Star Tick: Assay Techniques and Baseline Data12

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Abstract
Of five techniques studied for assaying the biological activity of acaricides against 3-month-old nymphs of Amblyomma americanum (L.) (Food and Agriculture Organization, filter paper dip, filter paper residue, pipet, and teabag), the pipet method provided the best combination of precision and ease of use. This method (test specimens sealed inside a disposable pipet that hat been previously dipped in an acaricide-acetone solution and allowed to dry) was used to establish base-line dosage-mortality data for A. americanum for 14 acaricides. Amitraz, permethrin, chlorpyrifos, and lindane, in that order, were most toxic based on LD90 values. The remaining compounds ranked from most to least toxic were: carbaryl, stirofos. diazinon, coumaphos, dioxathion, phosmet, toxaphene, methoxychlor, ronnel, and malathion.

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