Estimation of Hydrodynamic Volume of Proteins Using High-Performance Size-Exclusion Chromatography and Intrinsic Viscosity Measurement: An Attempt At Universal Calibration

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Abstract
Hydrodynamic volume was estimated by using high-performance size-exclusion chromatography (SEC) with a silica-based column and intrinsic viscosity ([η]) measurement, for native proteins and those reduced in sodium dodecyl sulphate (SDS) or guanidine hydrochloride (Gu-HCl). In solutions of 6M Gu-HCl, proteins in their reduced state behaved hydrodynamically as randomly coiled linear homopolymers and exhibited good linearity between an

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