High Perform ance Liquid Chrom atographie Analysis o f Steroidal Saponins from Avena sativa L

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Abstract
Reversed phase high performance liquid chromatography offers an efficient and rapid method for analysis of steroidal saponins. Crude extracts from primary leaves of Avena sativa and isolated etioplasts therefrom have been resolved into four saponins (avenacosides) using a water-acetonitrile gradient system on RP-8 and monitoring the column effluent at 200 nm with an UV-detector. Detectability was found to be in the range of 50 ng avenacoside B and the detector response was linear up to 8 μg tested. The described method is applicable to studies on localization and physiology of Avena saponins during development of the primary leaf.

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