A STUDY OF CHLOROPHYLL FLUORESCENCE IN VIVO BY A FLOW METHOD

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Abstract
Abstract— A flow method has been devised for the study of fluorescence transients during the induction period of photosynthetic systems. The principle of transformation of time‐transients into space‐transients is quantitatively worked out and the characteristics and mode of operation of the apparatus is described and illustrated with experimental results.With this method spectroscopic and kinetic investigation of in vivo chlorophyll fluorescence have been undertaken. The fluorescence emission can be split into two componmts—a variable part and a constant part—with different spectral distributions. The excitation spectrum of the light induced fluorescence changes shows that the variable part is sensitized by system 11, whereas the constant part mainly sensitized by system II has a small component originating from system I. The light‐induced fluorescence change decays in darkness with half‐time of about 10‐2s; this decay is highly sensitive to the action of inhibitors of oxygen evolution.The flow method can be extended to measurements of oxygen, luminescence, absorption differences. It permits using high‐intensity analytic beams with no actinic action; it is well suited for the integration of low level signals.

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