Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins
- 28 November 2004
- journal article
- Published by Springer Nature in Nature
- Vol. 433 (7023) , 317-322
- https://doi.org/10.1038/nature03126
Abstract
Select members of the Wnt family of secreted glycoproteins have been implicated in inducing the myogenic determinant genes Pax3, MyoD and Myf5 during mammalian embryogenesis, but the mechanism of induction has not been defined. We describe an unexpected role for protein kinase A (PKA) signalling via CREB in this induction. Using a combination of in vitro explant assays, mutant analysis and gene delivery into mouse embryos cultured ex vivo, we demonstrate that adenylyl cyclase signalling via PKA and its target transcription factor CREB are required for Wnt-directed myogenic gene expression. Wnt proteins can also stimulate CREB-mediated transcription, providing evidence for a Wnt signalling pathway involving PKA and CREB. Our findings raise the possibility that PKA/CREB signalling may also contribute to other Wnt-regulated processes in embryonic patterning, stem cell renewal and cancer.Keywords
This publication has 25 references indexed in Scilit:
- Wnt-frizzled signaling to G-protein-coupled effectorsCellular and Molecular Life Sciences, 2004
- Interplays of Gli2 and Gli3 and their requirement in mediating Shh-dependent sclerotome inductionDevelopment, 2003
- The Wnt signalling pathwayJournal of Cell Science, 2002
- The Promise and Perils of Wnt Signaling Through β-CateninScience, 2002
- The Wnt/Ca2+ pathwayTrends in Genetics, 2000
- CREB: A Stimulus-Induced Transcription Factor Activated by A Diverse Array of Extracellular SignalsAnnual Review of Biochemistry, 1999
- Frizzled proteins constitute a novel family of G protein-coupled receptors, most closely related to the secretin familyTrends in Pharmacological Sciences, 1998
- Combinatorial signaling by Sonic hedgehog and Wnt family members induces myogenic bHLH gene expression in the somite.Genes & Development, 1995
- MyoD or Myf-5 is required for the formation of skeletal musclePublished by Elsevier ,1993
- Mutational analysis of mouse Wnt-1 identifies two temperature-sensitive alleles and attributes of Wnt-1 protein essential for transformation of a mammary cell line.Molecular Biology of the Cell, 1992