Complete Nucleotide Sequence and Tissue‐Specific Expression of the Rat Phenylethanolamine N‐Methyltransferase Gene

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Abstract
The rat phenylethanolamine N‐methyltransferase (PNMT) gene was isolated from a genomic library by cross‐hybridization with a bovine PNMT cDNA probe. Complete nucleotide sequence analysis of a genomic clone showed that this gene contained three exons and spanned about 2.8 kb in length. There were the acute‐phase response element, TATA, SP1, and GRE sequences. The physicochemical properties of rat adrenal PNMT were different from those of the brainstem PNMT. However, northern blot and reverse transcription‐polymerase chain reaction analysis showed that the rat PNMT gene may not express the multiple forms of mRNA. These results suggest that the rat PNMT gene might produce a single enzyme protein, whose activity may be differentially modulated by tissue‐specific environment in the central and peripheral systems.

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