Evaluation of the genotoxic potential of zinc pyrithione in the Salmonella mutagenicity (Ames) assay, CHO/HGPRT gene mutation assay and mouse micronucleus assay
- 1 July 1993
- journal article
- research article
- Published by Wiley in Journal of Applied Toxicology
- Vol. 13 (4) , 283-289
- https://doi.org/10.1002/jat.2550130411
Abstract
The mutagenic potential of zinc pyrithione (Znpt) was evaluated in vitro in the Salmonella/mammalian microsome plate incorporation mutagenicity (Ames) assay and the CHO/HGPRT gene mutation assay. The clastogenic potential of Znpt was evaluated in vivo using the mouse micronucleus test. Znpt was negative in the Ames test in five tester strains in the presence and absence of rat liver microsomal enzymes when assayed at concentrations ranging between 10 and 333 μg per plate and between 0.03 and 33 μg per plate, respectively. Znpt also produced negative results in the CHO/HGPRT assay. No significant increases in mutant frequencies were seen in the presence and absence of rat liver microsomal enzymes. In each case, the highest concentrations reduced cellular viability by 83% and 85%, respectively. Znpt also did not induce increased frequencies of micronuclei in mouse bone marrow cells when tested at the maximally tolerated dose (MTD) (44 mg kg−1). These data support the conclusion that Znpt lacks genotoxic activity under the conditions of these tests.Keywords
This publication has 15 references indexed in Scilit:
- Revised methods for the Salmonella mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1983
- Mutagenic responses of five independent geentic loci in CHO cells to a variety of mutagensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1982
- The use of Chinese hamster ovary cells to quantify specific locus mutation and to determine mutagenicity of chemicals: A report of the GENE-TOX programMutation Research/Reviews in Genetic Toxicology, 1981
- Reproduction and teratology studies of zinc pyrithione administered orally or topically to rats and rabbitsFood and Cosmetics Toxicology, 1979
- A quantitative assay of mutation induction at the hypoxanthine-guanine phosphoribosyl transferase locus in Chinese hamster ovary cells (CHO/HGPRT system): Development and definition of the systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Urinary excretion and metabolism of salts of 2-pyridinethiol-1-oxide following intravenous administration to female Yorkshire pigsToxicology and Applied Pharmacology, 1976
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1975
- A percutaneous teratology study of zinc pyrithione in rabbitsToxicology and Applied Pharmacology, 1975
- Percutaneous Absorption and Besidual Amount on Skin Surface of Zinc bis (2-pyridylthio)-1, 1'-dioxide.Eisei kagaku, 1967
- The cause of blindness in dogs given hydroxypyridinethioneToxicology and Applied Pharmacology, 1962