Serum Albumin as a Food for Human Tubercle Bacilli

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Abstract
Dorset''s synthetic agar medium fortified with human serum albumin was comparable to Corper''s egg-yolk medium in supporting growth of minute inocula of a freshly isolated strain of human tubercle bacilli. Growth from 10-7 mg. tubercle bacilli occurred within 3 weeks on the synthetic agar medium plus 0.5% serum albumin and on the egg-yolk medium; no growth from 10-1 mg. was detected after 7 weeks on the synthetic medium without serum albumin. Because of its physical and nutritive properties, this serum albumin medium should be an efficient plating medium for research in which the estimation of numbers of viable tubercle bacilli is desired.

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