The polarographic estimation of steroid hormones

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Abstract
A micro-method was described for the quantitative separation of urinary 17-ketosteroid extracts into 3 (alpha) -and 3(beta)-hydroxy fractions. The 17-ketosteroids in the separated fractions were estimated polarographically. Recovery of alpha- and beta-17-ketosteroids from pure solns. and urine extracts was satisfactory. The urinary excretion of alpha- and beta-17-ketosteroids was detd. in a series of 10 normal males and ten normal females, and the results compared with those obtained by other workers. It is concluded that the total 17-ketosteroids excreted by normal individuals may contain up to 15% of 3(beta)-hydroxy compounds, though values less than 10% are more general. There is no significant difference between the beta-17-ketosteroid excretion of males and females.