P-Lim, a LIM homeodomain factor, is expressed during pituitary organ and cell commitment and synergizes with Pit-1.
Open Access
- 28 March 1995
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (7) , 2720-2724
- https://doi.org/10.1073/pnas.92.7.2720
Abstract
A pituitary LIM homeodomain factor, P-Lim, is expressed as Rathke's pouch forms and as specific pituitary cell phenotypes are established, suggesting functional roles throughout pituitary development. While selectively expressed in both anterior and intermediate pituitary in mature mice, P-Lim is also transiently expressed in the developing ventral neural cord and brainstem. P-Lim binds to and activates the promoter of the alpha-glycoprotein subunit gene, a marker of early pituitary development, and synergizes with Pit-1 in transcriptional activation of genes encoding terminal differentiation markers. The LIM domain of P-Lim specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.Keywords
This publication has 37 references indexed in Scilit:
- Activation of the glycoprotein hormone alpha-subunit promoter by a LIM-homeodomain transcription factor.Molecular and Cellular Biology, 1994
- LH-2: a LIM/homeodomain gene expressed in developing lymphocytes and neural cells.Proceedings of the National Academy of Sciences, 1993
- Synergistic activation of the insulin gene by a LIM-homeo domain protein and a basic helix-loop-helix protein: building a functional insulin minienhancer complex.Genes & Development, 1992
- Anterior pituitary development: Short tales from dwarf miceCell, 1992
- Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.Proceedings of the National Academy of Sciences, 1992
- The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos.Genes & Development, 1992
- Characterization of three RXR genes that mediate the action of 9-cis retinoic acid.Genes & Development, 1992
- Thyrotropin-releasing hormone regulation of human TSHB expression: role of a pituitary-specific transcription factor (Pit-1/GHF-1) and potential interaction with a thyroid hormone-inhibitory element.Proceedings of the National Academy of Sciences, 1991
- Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferaseAnalytical Biochemistry, 1991
- The pituitary-specific transcription factor GHF-1 is a homeobox-containing proteinCell, 1988