The Primary Structure of theαChain of a Mitogenic Lectin from the Seeds ofLathyrus sativus
- 1 January 1983
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 364 (2) , 1047-1052
- https://doi.org/10.1515/bchm2.1983.364.2.1047
Abstract
The complete primary structure of the .alpha. chain of the glucose- and mannose-specific lectin from L. sativus seeds was determined. The structure was elucidated by Edman degradation on the whole chain as well as on fragments derived from cleaving the chain with BNPS-skatole [3-bromo-3-methyl-2-(2-nitrophenylthio)-3H-indole] and of peptides derived after tryptic digestion of the citraconylated chain. The analyses revealed a heterogeneity in the chain length (53 or 55 residues) and a microheterogeneity in positions 47 (aspartic acid and glycine). The sequence was highly homologous to the .alpha. chain of the lectin from L. odoratus.This publication has 7 references indexed in Scilit:
- Interactions of Purified Human Ceruloplasmin withLathyrus odoratus, Lern culinarisandCanavalia ensiformisLectinsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Purification and properties of a mitogenic lectin from Lathyrus sativus seedsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Amino acid sequence and variant forms of favin, a lectin from Vicia faba.Journal of Biological Chemistry, 1982
- The structure of the lentil (Lens culinaris) lectin. Amino acid sequence determination and prediction of the secondary structure.Journal of Biological Chemistry, 1981
- The complete amino acid sequence of a prototype immunoglobulin-λ light-chain-type amyloid-fibril protein ARBiochemical Journal, 1981
- [34] Modification of tryptophan with BNPS-skatole (2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine)Published by Elsevier ,1972
- THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551Biochemical Journal, 1963