Direct activation of RNA polymerase III transcription by c-Myc
- 1 January 2003
- journal article
- Published by Springer Nature in Nature
- Vol. 421 (6920) , 290-294
- https://doi.org/10.1038/nature01327
Abstract
The proto-oncogene product c-Myc has a direct role in both metazoan cell growth and division. RNA polymerase III (pol III) is involved in the generation of transfer RNA and 5S ribosomal RNA, and these molecules must be produced in bulk to meet the need for protein synthesis in growing cells. We demonstrate here that c-Myc binds to TFIIIB, a pol III-specific general transcription factor, and directly activates pol III transcription. Chromatin immunoprecipitation reveals that endogenous c-Myc is present at tRNA and 5S rRNA genes in cultured mammalian cells. These results suggest that activation of pol III may have a role in the ability of c-Myc to stimulate cell growth.Keywords
This publication has 31 references indexed in Scilit:
- Deconstructing Myc: Figure 1.Genes & Development, 2001
- The RNA polymerase III transcription apparatus11Edited by P. E. WrightJournal of Molecular Biology, 2001
- Regulation of RNA Polymerase III Transcription during Cell Cycle EntryJournal of Biological Chemistry, 2001
- Differential activity of conditional MYC and its variant MYC-S in human mortal fibroblastsOncogene, 2000
- Cell growth: Downstream of Myc – to grow or to cycle?Current Biology, 1999
- The Myc oncoprotein: a critical evaluation of transactivation and target gene regulationOncogene, 1999
- The role of c-myc in cellular growth controlOncogene, 1999
- Repression of RNA polymerase III transcription by the retinoblastoma proteinNature, 1996
- Upregulated expression of the genes encoding translation initiation factors eIF-4E and eIF-2α in transformed cellsCancer Letters, 1996
- Binding and Suppression of the Myc Transcriptional Activation Domain by p107Science, 1994